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Holotoxin delivery and cell viability (CellTiter-Glo assay) 6×His-tagged proteases fused to the BoNT translocation domain via a thrombin-cleavable linker were expressed and purified as described above

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Subsequent linear regression analysis of these data points yielded the equation y = 0.9949x + 1.7722, where “y” denotes the GPx activity measured by the GPx-Tiron method and “x” signifies the GPx activity determined by the GPx-DTNB method

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